Detection of Metallo-Beta-lactamase Production in Rare Carbapenem-Resistant Non-fermentative Gram-Negative Bacilli Isolated in a Tertiary Care Hospital, Visakhapatnam, India
نویسندگان
چکیده مقاله:
Introduction: Non-fermentative Gram-negative bacilli (NFGNB) are occasionally involved in infectious diseases pathology, but have shown resistance to multiple antibiotics and the capability to gain new resistance factors in the hospital environment. The present study was aimed to investigate the antibiotic susceptibility pattern of rare NFGNB isolated from different clinical samples and the prevalence of Metallo-beta-lactamase (MBL) producing non-fermenters among the carbapenem-resistant isolates. Methods: A total of 250 clinical samples from the patients suffering from various infections were analyzed by using different standard microbiological techniques like microscopy, culture methods, biochemical reactions and antibiotic susceptibility using Kirby-Bauer method. MBL detection was performed by imipenem-EDTA combined disc test and imipenem-EDTA double disc synergy test (DDST). Results: The non-fermenters bacteria rate isolated from different clinical samples was 4.8%. The highest rate of non-fermentative isolates was observed in patients with hospital-acquired infections (91.6%). The various species of NFGNB included Pseudomonas putida (33.3%), Pseudomonas stutzeri (25%), Burkholderia cepacia (16.6%), Achromobacter xylosoxidans (16.6%) and Ochrobactrum anthropic (8.33%). The isolates showed high resistance to carbapenems, and the incidence of MBL producing non-fermenters among the carbapenem-resistant organisms was found to be 100%. Conclusion: NFGNB are now emerging as organisms of nosocomial infections. The use of broad spectrum antibiotics should be avoided, and quick detection and efficient infection control measures are essential to prevent further spread of MBLs to other Gram-negative bacilli. Detection of MBL production and rationale antibiotic usage are the most important factors which control the gradually increasing NFGNB related infections.
منابع مشابه
Phenotypic detection of carbapenem-susceptible metallo-beta-lactamase-producing gram-negative bacilli in the clinical laboratory.
Rapid detection of metallo-beta-lactamase (MBL)-producing gram-negative pathogens is critical to prevent their widespread dissemination. Thus far, no standardized phenotypic method is available, and previously reported techniques have poor sensitivity for detecting carbapenem-susceptible MBL-carrying isolates, an increasingly described phenomenon. We developed a phenotypic detection method usin...
متن کاملDetection of Carbapenem Resistant Gram-Negative Bacteria in Clinical Isolates from a Tertiary Care Hospital
Multidrug-resistant Gram-negative bacteria are a major public health threat. However, intense efforts to limit their spread, the number of multidrug resistant Gram-negative bacteria continues to increase globally. Carbapenems, the most broad-spectrum beta lactam antibiotics active against Gram-negative organisms, are very slowly hydrolyzed by most beta-lactamases. Because of their broad-spectru...
متن کاملScreening of Extended Spectrum Beta lactamase Producing Gram Negative Bacilli Isolated from Clinical Cases
Abstract Background and objectives: (ESBL) strain is one of the emerging health related problems in the world recently. Some of the species of the gram-negative bacilli including Klebsiella Pneumonia & Escherichia Coli are well known ESBL producing among bacteria, and they cause uncontrollable infections. This Cross-sectional study was designed to asses the ESBL producing gra...
متن کاملTreatment and Outcome of Carbapenem-Resistant Gram-Negative Bacilli Blood-Stream Infections in a Tertiary Care Hospital.
CONTEXT Infections caused by carbapenem-resistant bacteria constitute a major challenge for current medical practice. OBJECTIVE To describe treatment and outcome of carbapenem-resistant Gram-negative bacilli (GNB) blood-stream infection (BSI) caused by these organisms at a tertiary care hospital in Mumbai. METHODS Carbapenem-resistant isolates from blood cultures were collected from January...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 4 شماره 1
صفحات 31- 36
تاریخ انتشار 2016-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023